The mean diameter of seminiferous tubules was estimated from 30 randomly chosen round tubules in each testis, independent of their cycle stage. The majority of the seminiferous tubules are lined only by sertoli cells. Morphometric studies on rat seminiferous tubules deep blue. Pdf morphometric evaluation of seminiferous tubule and.
The postnatal morphologic and morphometric characteristics of donkeys seminiferous epithelium during the postnatal period were studied. Jul 01, 2002 also, the values found for seminiferous tubule volume density, tubular diameter, and total length of seminiferous tubule were significantly higher in donkeys p morphometric data in donkeys and mules mean sem. Figure 3 testis, germinal epithelium atrophy in a male f344n rat from a subchronic study. The testis was fixed in alcoholic bouin for 24 hours, and then process for.
Due to the smaller testis size, lower seminif erous tubule volume density, and fewer germ cells, the total length of seminiferous tubules in mules was significantly. The volume density of the seminiferous epithelium, lumen of tubules and the testis interstitium in the shampinealectomized adult wistar rats was determined after melatonin treatment and wholebody irradiation with 8 gy of gamma rays produced by 60 co. However, our study showed lower values than those reported for pantaneira 37. Overall, spermatozoa of seminiferous tubule had lower p morphometric abstract. Morphometric evaluation of seminiferous tubule and. Histopathological and histometrical assessment of boron. Spermatogenesis occurs in the seminiferous tubules, of which there are 1020 in each rat. Figure 3 testis normal in a male spraguedawley rat. No difference was observed in relation to the total length of seminiferous tubules g239. Seminiferous tubules coiled tubules within lobules site of sperm production myoid cells. The volume percentage of the tubular compartment seminiferous tubules was about 12.
The goal of this morphometric study was to obtain quantitative information on the seminiferous tubules of spraguedawley rats, including changes seen at various stages of the cycle of the seminiferous epithelium. No 46,xy spermatogonia were found in the seminiferous tubule sections of the 12 men with klinefelters syndrome who were negative for round spermatids and spermatozoa in the minced testicular samples. Curiously, no morphological or morphometric alterations were observed in animals treated with captopril or bpp11e 14. Morphometric evaluation of spermatogenic cells and seminiferous tubules and exploration of luteinizing hormone beta polypeptide in testis of datong yak qudratullah kalwar 1,2, min chu 1, anum ali ahmad 1,3, xuezhi ding 1, xiaoyun wu 1, pengjia bao 1 and ping yan 1. Structure and gametogenic potential of seminiferous tubules in ageing mice. Every complete seminiferous tubule profile in a section usually 510 was included in the following analyses. Among them, op inhibit dna synthesis in the seminiferous epithelium. Seminiferous tubules cross sections were randomly chosen in three nonserial sections per animal, totalizing 100 tubules animal. Morphometric aspects of seminiferous tubules in rats treated. Histological examination of testes is essential for understanding infertility, sex development, and growth. Morphometric evaluation of seminiferous tubule and proportionate numerical analysis of sertoli and spermatogenic cells indicate differences between crossbred and purebred bulls may 2015 veterinary. Pdf morphometric evaluation of seminiferous tubules in. The objective of this study was to determine the testicular volume parameters and correlate data from morphometry of testis and seminiferous tubules with body. This study aimed to assess the biometrics of the testes and the morphology of the seminiferous tubules of crioulo horses.
The expected protective role of celery oil on induced toxic. Morphometric evaluation of seminiferous tubules in aged mice. Testicular morphology and stereological evaluation of the seminiferous. Dec 31, 20 the seminiferous tubules of all study groups with changes in epithelial height at various sacrifice times are shown in figures 3, 4 and 5. Pdf stereological and morphometric analysis of collagen. The seminiferous tubules diameters were not statistically significant different between the testes of patients treated and nontreated with hcg 67. Morphology and morphometry of seminiferous tubules in. Pdf stereological and morphometric analysis of collagen and.
Morphometric evaluation of seminiferous tubules from each section, seminiferous tubule diameter and area essentially from circular tubular cross sections was determined using precalibrated measuring eyepiece. The percentage of germinal epithelium in the seminiferous tubules table 1 was higher in boars p seminiferous tubules table 1 was higher in immunocastrates p morphometric analysis of collagen and seminiferous tubules in testes of patients with cryptorchidism submitted or not to treatment with human chorionic gonadotrophin luciano a. Citeseerx document details isaac councill, lee giles, pradeep teregowda. Histological techniques, morphometric analysis of seminiferous tubules and sperm count were performed on testes and cauda epididymides from both groups, th histological techniques. Mar 01, 2017 crosssections of seminiferous tubules characterizing stages 5 a, 6 b, 7 c, and 8 d of the seminiferous epithelium of orchiectomied crioulo horses during nonreproductive season. Taking into account that the epithelial area can be altered without to affect, necessarily, the total tubular area, these two areas were evaluated. Wild boars sus scrofa scrof a seminiferous tubules morphometry. Seminiferous tubules of immature males are small and lined by a single layer of cells. Test values of the correlation tests between the lp components confirm the location of these alterations within its internal layer. The expected protective role of celery oil on induced. Seminiferous tubule and leydig cell volume density % in cats were approximately 90% and. The goal of this morphometric study was to obtain quantitative information on the seminiferous tubules of sprague. The experiment was conducted on 40 adult aval medical n esearch institute mice.
Figure 4 testis, germinal epithelium atrophy in a male f344n rat from a chronic study. This study was carried out to identify testicular biometric data of native egyptian buffalobulls through the determination of testicular parameters by using ultrasound examination of the testis and scrotum and determination of scrotal circumference of the life animal and morphometric data of the testis after. The length of seminiferous tubule per gram of testis is estimated to be 12. Retention of elongated spermatids in the basal region of a stage xii seminiferous tubule. Pdf some morphometric and histological description of. Structural alterations in the seminiferous tubules of rats. The current study aims to add further granularity to these observations by describing alterations in the expression pattern of multiple. Camel epididymis seminiferous tubules sertoli cells. The mean diameter of the seminiferous tubules was 205. The spermatogonial mitoses efficiency coefficient, meiotic index and spermatogenesis efficiency were 10.
Scrotal circumference a and morphometric parameters of the seminiferous tubules b, c, d with high red bars and low blue bars plasma testosterone concentration. This page introduces spermatogenesis the development of spermatozoa, the male haploid gamete cell. Seasonal variations of the seminiferous tubules morphometry. In animals that were developed enough for the tubular lumen to be recognizable, the height of the seminiferous epithelium was determined from 10 round tubules at stages vii and viii, which. The mean diameter of the seminiferous tubules was 150. Morphology of mouse seminiferous tubules springerlink. Original article morphometric evaluation of seminiferous tubules in aged mice testes after melatonin administration fereshteh mehraein, ph. Morphometric analysis of mice testicular tubules after administration of. Comparative testis morphometry and seminiferous epithelium. Stereological and morphometric analysis of collagen and seminiferous tubules in testes of patients with cryptorchidism submitted or not to treatment with human chorionic gonadotrophin.
Histological and morphometric examination of the testes of. The testis of transgenic mice indicated many focal damages, in which some seminiferous tubules contained mostly spermatogenic cells while others were very. Morphometry in seminiferous tubules and ductus epididymis the mean diameter of seminiferous tubules was estimated from 30 randomly chosen round tubules in each testis, independent of their cycle stage. Involution of seminiferous tubules in aged hamsters. Feb 01, 2021 spermatozoa n 7010 collected from different regions seminiferous tubules. Sperm head morphometry and chromatin condensation are in.
Testes from 8 adult rats were perfusionfixed with buffered glutaraldehyde and semithin sections of the seminiferous epithelium at all stages of the spermatogenic cycle were subjected to morphometric analysis at the light microscope level. In group e3 a severe histological damage predominated. In the seminiferous tubules of the latter men, only 47,xxy sertoli. Material and methods five adult european wild boars 12. A morphometric study for each chosen seminiferous tubule that included external seminiferous tubular diameter std and seminiferous epithelial height seh were measured by olysia bio software. This is also true for primary spermatocytes of various.
Morphometric measures in seminiferous tubules with and without granular sertoli cells were obtained. Morphological and morphometric study of early changes in. In this investigation, testes of 20 donkeys ranging from birth to maturity were studied. Toxicologic pathology histological development of male the. Almost all of the seminiferous tubules were shrunken and hyalinized. Presently, we do not have any published information on morphology biometry of seminiferous tubule, spermatogenic cells and sertoli cells in crossbred bulls and. A light microscopic and morphometric analysis of the. Tunica albuginea, note the arteriole branches between intensive collagen fibers a rrow, s seminiferous tubule. In the seminiferous tubules of the latter men, only 47,xxy sertoli cells and 47,xxy germ cells were seen figure 3.
Morphometric analysis of leydig cells in the normal rat. Twenty seminiferous tubules in stage vix were measured in each section. A second process of spermiogenesis leads to change in cellular organisation and shape before release into the central lumen of the seminiferous tubule. Toxicological effects of bioactive peptide fractions obtained. Pdf morphometric evaluation of seminiferous tubules in aged. These data have suggested that the alterations in the structure and function of the seminiferous epithelium in mice are dependent on their primary molecular. Figure 2 testis, seminiferous tubule retention, spermatid in a male spraguedawley rat. Granular changes in sertoli cells in children and pubertal patients. In humans at puberty, spermatozoa are produced by spermatogonia meiosis in the seminiferous tubules of the testis male gonad. Morphometric evaluation of seminiferous tubule and proportionate.
Within the seminiferous tubules, cells are associated in an organized and predetermined architecture, with a wall composed by connective tissue on which the seminiferous epithelium rests tchernitchin, 1995. Morphometric analysis of mice testicular tubules after. Di 2ethylhexyl phthalate, testis, sperm, morphometry. In animals that were developed enough for the tubular lumen to be recognizable, the height of the seminiferous epithelium was deter. Sep 01, 1982 the goal of this morphometric study was to obtain quantitative information on the seminiferous tubules of spraguedawley rats, including changes seen at various stages of the cycle of the seminiferous epithelium.
From our morphometric results, corrected for volume changes during preparation for microscopy, there are 15. Rynchotus rufescens partridge was investigated, analyzing the testis weight, the seminiferous tubules diameter, the thickness of the. Some apparently normal seminiferous tubules were present among tubules with dis organized germinal epithelium and multinucleat cells or showing only remnant sertoli cells. Samples of parenchymal tissue of cryptorchid testis. Pediatric urology testis histology in patients treated or not with hcg international braz j urol vol. Spss software and students ttest analyzed all parameters to assess the significance of changes between control and ex. Morphometric study a morphometric study for each chosen seminiferous tubule that included external seminiferous tubular diameter std and seminiferous epithelial height seh were measured by olysia bio software. Streological and morphometric analysis of collagen and seminiferous tubules in testes of patients with cryptorchidism submitted or not to treatment with human chorionic gonadotrophin. Using this method, we examined seminiferous tubules from normal mice. Histological and anatomical study of the white rooster of. Developmental study of phathalate effects on seminiferous tubules. Seminiferous tubular diameter std the meanse of external diameter of seminiferous tubules was 276. Testicular morphometry and histology of rabbit bucks. Pdf histological and morphometric analysis of testes of adult.
Tissue from perfusionfixed testes was embedded in eponaraldite. Each cubic centimeter contained approximatelyy 1 g of rat testis contained about 22 million leydig cells. The weight of birds and testes, volume, length, circumference, seminiferous tubule diameter, circumference and height of germinal epithelium of. Kent christensen department of anatomy and cell biology, the university of michigan medical school, ann arbor, michigan 48109 abstract the goal of this morphometric study was to obtain quantitative information on the seminiferous tubules of sprague. Pdf morphological and morphometric study of the development. Morphometric studies on rat seminiferous tubules wing 1982. The stage of the cycle of the seminiferous epithelium seen in the profile.
The interstitial connective tissue thickened significantly in the experimental group p 0. Morphometric and cytogenetic characteristics of testicular. In the present study, we performed a comparative and more accurate morphometric and functional investigation of the testis in donkeys and mules. Morphometry of testis and seminiferous tubules of the adult. Therefore, to understand the histomorphology of testes at different developmental stages, we performed hematoxylin and eosin staining of yak testis. Wild boars sus scrofa scrof a seminiferous tubules. A light microscopic and morphometric analysis of the sertoli. Quantify the distribution of collagen and analyze the seminiferous tubules diameter in the testis of patients with cryptorchidism, to verify if the previous use of human chorionic gonadotrophin hcg affects these structures.
Stereological and morphometric analysis of collagen and. Discussion regarding seminiferous tubular diameter std, ribavirin caused decrease in std at all sacrifice times. Morphological and morphometric study of early changes in the. The frequency of leydig cells, the thickness of tunica albuginea and the cross sectional lengths and breadths of the seminiferous tubules were compared between groups by using student.
Fot the morphometric measurements the functions of the analysis pro software measure module were used. The number of cell nuclei b, tubular area c, tubular diameter and the number of cell nuclei b, tubular area c, tubular diameter and seminiferous epithelium height d were measured with the leica qwinpro software bar scale50m. Six days after the last injection, all mice weresacrificed and the testes were excised and processed for light microscope observation. The right and left testicles were found to be oval in shape, and placed to the left and right sides of midline. The quantitative histology of the testicles, efficiency of leydig and sertoli cells, and general morphometry of the seminiferous tubule may be. Morphometric findings on testes showed tcdd treatment led to the loss of germ cells and decreased spermatogenesis cell lines, reducing epithelium and diameter of the seminiferous tubules fig. Fertility of the monorchid rat after partial resection of testes. The males were divided into five age groups based on months of age. Toxicological effects of bioactive peptide fractions. May 01, 2003 in general, 1015 m of seminiferous tubules are found per gram of testis parenchyma 11, 33. Percentage of total seminiferous tubules containing different phenotypes and germ cell. Control stage xi tubule for comparison with figure 1. Testis morphometry, seminiferous epithelium cycle length. Results melatonintreated mice had seminiferous tubules with a wide lumen lined by low height germinal epithelium.
The r control group was located in the center of the i bore while it was turned off, while mr the exposed group was exposed to the active scanner for 36 minutes once a week for three weeks. Morphometric studies of the leydig cells in elderly men with special reference to the histology of the prostate. Morphometric evaluation of seminiferous tubules in aged. Ultrastructural data of lysosomes in human and rat sertoli. Data obtained for the morphometric characteristics were subjected to analysis of variance anova. Results and discussion at the specimens from the age of 120 days in the seminal epithelium are. In the morphometric study, we evaluated testicular seminiferous tubule parameters suchas height of germinal epithelium, seminiferous tubule diameter, thickness of interstitialconnective tissue and spermatogenesis. Morphometric results the morphometric findings are depicted in table 1. The length of seminiferous tubules per gram of testis in crioulo horses 26.
The quantity of spermatogenic cells and sperm quality in male reproductive system was affected by toxic substance 2methoxyethanol 2me. In seminiferous tubules, the seminiferous epithelium is formed by two types of cells. The number of sertoli cells is constant per unit length of the seminiferous tubule at all stages of the cycle. Due to the smaller testis size, lower seminiferous tubule volume density, and fewer germ cells, the total length of seminiferous tubules in mules was significantly smaller than in donkeys. However, probably due to the fact that the tubular diameter in domestic cats is not high and that almost 90% of seminiferous tubules are found in testis parenchyma, approximately 20 m of tubules per gram are observed in this species. Morphometric studies on rat seminiferous tubules wing. Morphometry of seminiferous tubules 15 ments for each animal was taken as thevolume density of the structure in the animal. Methods are presented to derive the volume occupied by the sertoli cells within the total volume of the seminiferous epithelium at each stage of the cycle. Anatomy department, medical school, tehran university of medical sciences, tehran, iran corresponding address. Morphometric evaluation of spermatogenic cells and seminiferous. Testis morphometry, seminiferous epithelium cycle length, and. Morphology and morphometry of seminiferous tubules in crioulo.
741 1076 197 1136 919 1461 1483 185 487 1143 993 1170 439 1249 245 904 1133 1461 700 1484 1041 822 1460 995 645 439